In defense of Copi
نویسندگان
چکیده
منابع مشابه
COPI-independent Anterograde Transport: Cargo-selective ER to Golgi Protein Transport in Yeast COPI Mutants
The coatomer (COPI) complex mediates Golgi to ER recycling of membrane proteins containing a dilysine retrieval motif. However, COPI was initially characterized as an anterograde-acting coat complex. To investigate the direct and primary role(s) of COPI in ER/Golgi transport and in the secretory pathway in general, we used PCR-based mutagenesis to generate new temperature-conditional mutant all...
متن کاملThe Cytoplasmic Coatomer Protein COPI
Expression of the asialoglycoprotein receptor (ASGR) by the human hepatocellular carcinoma cell lines HepG2 and HuH-7 in response to intracellular cGMP concentrations was previously shown to be regulated at the translational level (1). Stable transfection of COS-7 cells with deletion constructs encoding the asialoglycoprotein receptor H2b subunit localized the cGMPresponsive cis-acting element ...
متن کاملDoes COPI Go Both Ways?
from one Golgi complex to another under conditions that * Department of Molecular Cell Biology mirrored the conditions required for vesicle formation, Howard Hughes Medical Institute docking, and fusion. However, to many investigators University of California in the field, it remained uncertain whether the vesicles Berkeley, California 94720-3202 represented an obligatory intermediate in intra-...
متن کاملSpatiotemporal dynamics of the COPI vesicle machinery.
Assembly of the coat protein I (COPI) vesicle coat is controlled by the small GTPase ADP ribosylation factor 1 (ARF1) and its GTPase-activating protein, ARFGAP1. Here, we investigate the diffusional behaviours of coatomer, the main component of the coat, and also those of ARF1 and ARFGAP1. Using fluorescence-correlation spectroscopy, we found that most ARF1 and ARFGAP1 molecules are highly mobi...
متن کاملThe Energy of COPI for Budding Membranes
As a major actor of cellular trafficking, COPI coat proteins assemble on membranes and locally bend them to bud 60 nm-size coated particles. Budding requires the energy of the coat assembly to overcome the one necessary to deform the membrane which primarily depends on the bending modulus and surface tension, γ. Using a COPI-induced oil nanodroplet formation approach, we modulated the budding o...
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ژورنال
عنوان ژورنال: Notre Dame Journal of Formal Logic
سال: 1975
ISSN: 0029-4527
DOI: 10.1305/ndjfl/1093891909